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Dr. Ariel Waisman

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I lead a dynamic and multidisciplinary research group focused on cardiac regeneration using human pluripotent stem cells. Our goal is to decipher mechanisms that enable human heart regeneration and develop innovative therapeutic strategies.

My current team:

  • Doctoral students: Joaquín Smucler, Denisse Saulnier, Julia Halek
  • Undergraduate students: Agustín Aguzzi, Theo Fernández Long
  • Upcoming doctoral candidate: Joaquín Etcheparreborda

Research Lines

Cardiac Differentiation and Maturation

We study the differentiation of human pluripotent stem cells into cardiomyocytes, with particular emphasis on maturation processes that transform cardiomyocytes from a fetal to a postnatal phenotype. This transition is key to developing relevant models of the adult human heart.

Mature Cardiomyocyte Proliferation

We investigate strategies to induce proliferation of mature cardiomyocytes through the expression of specific factors using advanced methodologies such as baculovirus transduction. In parallel, we develop specialized tools to accurately quantify proliferation rates, overcoming the technical challenges associated with the polynucleation and polyploidy characteristic of these cells.

Applied Technologies

My laboratory integrates cutting-edge technologies:

  • Cardiac differentiation of human pluripotent stem cells
  • Advanced image analysis and proprietary computational tools
  • CRISPR homologous recombination to generate fluorescent reporter lines
  • Photolithography-generated micropatterns for cellular microenvironment control

Selected publications

Multi-Channel Nuclear Analysis: An ImageJ/FIJI Plugin for Automated Nuclear Segmentation and Multi-Channel Fluorescence Analysis

Ariel Waisman
microPublication biology, 2025


The transcription factor OCT6 promotes the dissolution of the naïve pluripotent state by repressing Nanog and activating a formative state gene regulatory network

Ariel Waisman, Federico Sevlever, Denisse Saulnier, Marcos Francia, Renata Blanco, Guadalupe Amín, Antonella Lombardi, Celeste Biani, María Belén Palma, Agustina Scarafía, Joaquín Smucler, Alejandro La Greca, Lucía Moro, Gustavo Sevlever, Alejandra Guberman, Santiago Miriuka

Scientific Reports, 2024


Automatic and unbiased segmentation and quantification of myofibers in skeletal muscle

Ariel Waisman, Alessandra Marie Norris, Martín Elías Costa, Daniel Kopinke

Scientific Reports, 2021


Deep Learning Neural Networks Highly Predict Very Early Onset of Pluripotent Stem Cell Differentiation

Ariel Waisman, Alejandro La Greca, Alan M Möbbs, María Agustina Scarafía, Natalia L Santín Velazque, Gabriel Neiman, Lucía N Moro, Carlos Luzzani, Gustavo E Sevlever, Alejandra S Guberman, Santiago G Miriuka

Stem Cell Reports, 2019


Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency

Ariel Waisman, Federico Sevlever, Martín Elías Costa, María Soledad Cosentino, Santiago G Miriuka, Alejandra C Ventura, Alejandra S Guberman

Scientific Reports, 2019


Inhibition of cell division and DNA replication impair mouse-naive pluripotency exit

Ariel Waisman, Camila Vazquez Echegaray, Claudia Solari, María Soledad Cosentino, Iain Martyn, Alessia Deglincerti, Mohammad Zeeshan Ozair, Albert Ruzo, Lino Barañao, Santiago Miriuka, Ali Brivanlou, Alejandra Guberman

Journal of molecular biology, 2017